![]() Adaptor complementary sequences on the bead’s surface aid in amplification on the bead. The fragments are mixed with small beads, PCR reagents and DNA polymerases. After that, things get a little different.Īmplifying DNA fragments is done by emulsion PCR, which counts and dilutes the library fragments. Instead of optically reading dye-labeled nucleotides, Ion Torrent detects electrical signals on a semiconductor chip.įor the Ion Torrent PGM and Proton machines, library construction is like other sequencing processes, including DNA fragmentation, end polishing with enzymes, and ligation of adapters. Ion Torrent sequencing is based on a unique technology. These were benchtop machines that showed their prowess in quickly sequencing smaller exomes and other DNA samples (about 10-20 million bases per run, compared to Illumina HiSeq, which could read 250 billion bases in a run). Ion Torrent technology, when it was introduced in 2010, was one of several machines that promised to revolutionize genetics. ![]()
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